Journal: PLoS ONE

Article Title: Potentiation of Epidermal Growth Factor-Mediated Oncogenic Transformation by Sialidase NEU3 Leading to Src Activation

doi: 10.1371/journal.pone.0120578

Figure Lengend Snippet: (A) The cell growth curves of NEU3-transfected cells were compared with those of vector controls in the absence and presence of murine EGF (20 ng/ml). Three independent experiments were performed (mean ±SD). (B) The cell growth curves of EGFR- and EGFR/NEU3-transfected cells are shown with or without EGF in independent experiments performed in triplicate (mean ±SD). (C) Colony formation assays in the transfectants. The cells were plated at 1000 cells/well in six-well dishes with or without EGF, and the colonies were quantified after 7–14 days of culture. Representative images are shown. (D) Values represent means with standard deviations obtained from three independent experiments. In the right graph, the number of colonies over 3.0 mm in size was counted in independent experiments performed in triplicate (mean ±SD).

Article Snippet: Mice were injected subcutaneously with the cells (1x10 7 ) under ether anesthesia and then given either subcutaneous doses of murine EGF (Nacalai Tesque, 5 μg/day) every two days or no additional treatment.

Techniques: Transfection, Plasmid Preparation

Journal: PLoS ONE

Article Title: Potentiation of Epidermal Growth Factor-Mediated Oncogenic Transformation by Sialidase NEU3 Leading to Src Activation

doi: 10.1371/journal.pone.0120578

Figure Lengend Snippet: (A) Anchorage-independent growth in soft agar. The cells (1 x 10 5 ) were plated on soft agar with or without EGF, and two to three weeks later, the colony number and size were measured. Representative images are shown. (B) In the graph, the colony number on soft agar was calculated. The values are means with standard deviations obtained from three independent experiments. The graph in the right indicates the number of colonies over 200 μm in size. (C) In vivo growth in nude mice. Respective cells were subcutaneously transplanted into nude mice with injection of murine EGF (5 μg/ml) every two days, and the measured tumor weights are indicated. The results of experiments with the vector controls and NEU3-transfected cells (left graph) and those with EGFR- and EGFR/NEU3-transfected cells (right graph) are shown in the four groups (n = 3 or 4). (D) Tumor incidence in the mice transplanted with the cells. To confirm the results, other six groups (n = 4) of these cells were further examined with or without injection of EGF, as indicated in the lower table.

Article Snippet: Mice were injected subcutaneously with the cells (1x10 7 ) under ether anesthesia and then given either subcutaneous doses of murine EGF (Nacalai Tesque, 5 μg/day) every two days or no additional treatment.

Techniques: In Vivo, Injection, Plasmid Preparation, Transfection